IFN-Gamma Secretion and IL-10 after Stimulation of ESAT-6-CFP-10 (EC610) Fusion Antigen in Patients with Active Tuberculosis and Latent Tuberculosis
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منابع مشابه
IFN-γ and IL-2 Responses to Recombinant AlaDH against ESAT-6/CFP-10 Fusion Antigens in the Diagnosis of Latent versus Active Tuberculosis Infection
Background: Discriminating latent tuberculosis infection (LTBI) from active TBI may be challenging. The objective of this study was to produce the recombinant L-alanine dehydrogenase (AlaDH) antigen and evaluate individuals with LTBI, those with active TBI, and uninfected individuals by enzyme-linked immunospot assay (ELISPOT) in order to distinguish LTBI from active TBI.Methods: This explorato...
متن کاملifn-γ and il-2 responses to recombinant aladh against esat-6/cfp-10 fusion antigens in the diagnosis of latent versus active tuberculosis infection
background: discriminating latent tuberculosis infection (ltbi) from active tbi may be challenging. the objective of this study was to produce the recombinant l-alanine dehydrogenase (aladh) antigen and evaluate individuals with ltbi, those with active tbi, and uninfected individuals by enzyme-linked immunospot assay (elispot) in order to distinguish ltbi from active tbi. methods: this explorat...
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Background: Macrophage immune responses are affected by the secretory proteins of Mycobacterium tuberculosis (Mtb). This study aimed to examine the immune responses of macrophages to Mtb secretory antigens, namely ESAT-6, CFP-10, and ESAT-6/CFP-10.Methods: THP-1 cells (a human monocytic cell line) were cultured and differentiated to macrophages by phorbol 12-myristate 13-acetate. The cytotoxici...
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Background: Tuberculosis is a disease with high morbidity, caused mainly by Mycobaterium tuberculosis (M.tb.). DNA vaccines show a promising future due to their unique advantages over conventional methods. The early-secreted antigen target (ESAT)-6 and culture filtrate protein (CFP)-10 of M.tb. antigens have been identified as vaccine candidates against Mycobacteria and used as subunit vaccines...
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IFN-gamma responses to Mycobacterium tuberculosis antigens ESAT-6 and CFP-10 have been proposed as specific markers of M. tuberculosis infection. Monokine induced by gamma interferon (MIG/CXCL9) has been shown to be expressed by IFN-gamma stimulated mononuclear cells and to attract activated T-cells through the chemokine receptor CXCR3. Since MIG is induced early in the response to IFN-gamma, m...
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ژورنال
عنوان ژورنال: Biomedical Journal of Indonesia
سال: 2019
ISSN: 2685-0184,2407-7097
DOI: 10.32539/bji.v5i3.8897